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rt2 sybr green qpcr master mix pa-110  (SuperArray Bioscience Corporation)

 
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    SuperArray Bioscience Corporation rt2 sybr green qpcr master mix pa-110
    SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by <t>qPCR</t> (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.
    Rt2 Sybr Green Qpcr Master Mix Pa 110, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt2+sybr+green+qpcr+master+mix+pa-110/pmc02823973-60-30-37?v=SuperArray+Bioscience+Corporation
    Average 90 stars, based on 1 article reviews
    rt2 sybr green qpcr master mix pa-110 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Sonic Hedgehog (SHH) Promotes the Differentiation of Mouse Cochlear Neural Progenitors via the Math1 -Brn3.1 Signaling pathway in vitro"

    Article Title: Sonic Hedgehog (SHH) Promotes the Differentiation of Mouse Cochlear Neural Progenitors via the Math1 -Brn3.1 Signaling pathway in vitro

    Journal:

    doi: 10.1002/jnr.22286

    SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by qPCR (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.
    Figure Legend Snippet: SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by qPCR (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.

    Techniques Used: In Vitro, Expressing, Immunohistochemistry, Activity Assay, Luciferase, Comparison

    Math1 activates the expression of Brn3.1 in CNPs. In comparison with ev transfection, Math1 cDNA transfection in CNPs successfully induced the expression of the Math1 mRNA transcripts at day 1 or 2 (A). Note that the higher the mRNA transcripts are, the lower the “threshold” cycles (Ct) are. Luciferase assays demonstrated that Math1 cDNA transfection in CNPs significantly increased the promoter activity of Brn3.1 in a time-dependent manner (B). The expression level of Brn3.1 mRNA transcripts was higher in those Math1-transfected CNPs than in ev-transfected CNPs for 2–4 days (C). Note that Math1-transfected CNPs have a remarkable increase of the mRNA transcripts for Math1 and a detectable increase of the mRNA for Brn3.1. Y axis, fluorescent intensity; X axis, cycle; solid line, the fluorescent level (approximately 200~500) being used as the “threshold” cycle of qPCR in this study (true threshold cycle at 100 or below).
    Figure Legend Snippet: Math1 activates the expression of Brn3.1 in CNPs. In comparison with ev transfection, Math1 cDNA transfection in CNPs successfully induced the expression of the Math1 mRNA transcripts at day 1 or 2 (A). Note that the higher the mRNA transcripts are, the lower the “threshold” cycles (Ct) are. Luciferase assays demonstrated that Math1 cDNA transfection in CNPs significantly increased the promoter activity of Brn3.1 in a time-dependent manner (B). The expression level of Brn3.1 mRNA transcripts was higher in those Math1-transfected CNPs than in ev-transfected CNPs for 2–4 days (C). Note that Math1-transfected CNPs have a remarkable increase of the mRNA transcripts for Math1 and a detectable increase of the mRNA for Brn3.1. Y axis, fluorescent intensity; X axis, cycle; solid line, the fluorescent level (approximately 200~500) being used as the “threshold” cycle of qPCR in this study (true threshold cycle at 100 or below).

    Techniques Used: Expressing, Comparison, Transfection, Luciferase, Activity Assay



    Similar Products

    rt2 sybr green qpcr master mix pa-110  (SuperArray Bioscience Corporation)
    90
    SuperArray Bioscience Corporation rt2 sybr green qpcr master mix pa-110
    SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by <t>qPCR</t> (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.
    Rt2 Sybr Green Qpcr Master Mix Pa 110, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt2+sybr+green+qpcr+master+mix+pa-110/pmc02823973-60-30-37?v=SuperArray+Bioscience+Corporation
    Average 90 stars, based on 1 article reviews
    rt2 sybr green qpcr master mix pa-110 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by qPCR (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.

    Journal:

    Article Title: Sonic Hedgehog (SHH) Promotes the Differentiation of Mouse Cochlear Neural Progenitors via the Math1 -Brn3.1 Signaling pathway in vitro

    doi: 10.1002/jnr.22286

    Figure Lengend Snippet: SHH increases the differentiation of CNPs in vitro. Addition of SHH to CNPs significantly increased the expression of the Math1 mRNA transcripts by qPCR (A), the percentage of Math1+ cells by FACS (B), and the expression of Math1 and myosin VIIa in cells by immunohistochemistry (C), and the promoter activity of the Math1 gene by luciferase assays (D). Note that cell starvation (PBS, omitting EGF, FBS, and SHH) also significantly increases the promoter activity of Math1 compared with untreated cells. In comparison with PBS (E) and MEM-treated (F), SHH increased the formation of epithelial cell islands (G). Bar in C=10 μM; *p<0.05, **p<0.05.

    Article Snippet: Cells were cultured in FGM for 2, 3, and 4 days and then harvested for qPCR experiments at the end of day 2, 3, and 4. qPCR was performed using RT2 SYBR Green qPCR Master Mix (PA-110, SuperArray) on Eppendorf Mastercycler® according to the manufacturer's instructions. qPCR data were analyzed by realplex (Eppendorf®) and plotted with Sigmaplot (SPSS Inc.) as previously described ( Zheng et al. 2008 ).

    Techniques: In Vitro, Expressing, Immunohistochemistry, Activity Assay, Luciferase, Comparison

    Math1 activates the expression of Brn3.1 in CNPs. In comparison with ev transfection, Math1 cDNA transfection in CNPs successfully induced the expression of the Math1 mRNA transcripts at day 1 or 2 (A). Note that the higher the mRNA transcripts are, the lower the “threshold” cycles (Ct) are. Luciferase assays demonstrated that Math1 cDNA transfection in CNPs significantly increased the promoter activity of Brn3.1 in a time-dependent manner (B). The expression level of Brn3.1 mRNA transcripts was higher in those Math1-transfected CNPs than in ev-transfected CNPs for 2–4 days (C). Note that Math1-transfected CNPs have a remarkable increase of the mRNA transcripts for Math1 and a detectable increase of the mRNA for Brn3.1. Y axis, fluorescent intensity; X axis, cycle; solid line, the fluorescent level (approximately 200~500) being used as the “threshold” cycle of qPCR in this study (true threshold cycle at 100 or below).

    Journal:

    Article Title: Sonic Hedgehog (SHH) Promotes the Differentiation of Mouse Cochlear Neural Progenitors via the Math1 -Brn3.1 Signaling pathway in vitro

    doi: 10.1002/jnr.22286

    Figure Lengend Snippet: Math1 activates the expression of Brn3.1 in CNPs. In comparison with ev transfection, Math1 cDNA transfection in CNPs successfully induced the expression of the Math1 mRNA transcripts at day 1 or 2 (A). Note that the higher the mRNA transcripts are, the lower the “threshold” cycles (Ct) are. Luciferase assays demonstrated that Math1 cDNA transfection in CNPs significantly increased the promoter activity of Brn3.1 in a time-dependent manner (B). The expression level of Brn3.1 mRNA transcripts was higher in those Math1-transfected CNPs than in ev-transfected CNPs for 2–4 days (C). Note that Math1-transfected CNPs have a remarkable increase of the mRNA transcripts for Math1 and a detectable increase of the mRNA for Brn3.1. Y axis, fluorescent intensity; X axis, cycle; solid line, the fluorescent level (approximately 200~500) being used as the “threshold” cycle of qPCR in this study (true threshold cycle at 100 or below).

    Article Snippet: Cells were cultured in FGM for 2, 3, and 4 days and then harvested for qPCR experiments at the end of day 2, 3, and 4. qPCR was performed using RT2 SYBR Green qPCR Master Mix (PA-110, SuperArray) on Eppendorf Mastercycler® according to the manufacturer's instructions. qPCR data were analyzed by realplex (Eppendorf®) and plotted with Sigmaplot (SPSS Inc.) as previously described ( Zheng et al. 2008 ).

    Techniques: Expressing, Comparison, Transfection, Luciferase, Activity Assay